AIP CONFERENCE PROCEEDINGS, vol.2803, no.1, pp.1-2, 2023 (Conference Book)
This study is intended to prepare smart functionalized AgNPs using biomimetic synthesis pathway and to evaluate their antimicrobial potential. For this purpose, visible light sensitive polysaccharide nanocomposite template was designed. Structure of visible light mediated AgNPs with size mean 3.5 nm was examined by UV- Vis and TEM Techniques. Antimicrobial activity and synergistic effect of AgNPs were evaluated against the following microorganisms: Enterococcus faecalis 29212, Escherichia coli 25922, Pseudomonas aeruginosa 27853, Candida albicans 10231 using the agar disk diffusion method. The antimicrobial analyses show effect in a dose-dependent manner: E.coli 25922 samples treated with the initial concentration of AgNPs (2,5 mg/mL) show the biggest inhibition zone diameter with 16 mm and up to 4 dilutions (0.05 mg/mL) AgNPs still show antibacterial potency within 7 mm inhibition zone. Recorded reduced cytotoxic effect at 0.05 mg/mL towards used bacteria and at 0.1 mg/mL against yeast verify that visible light synergistically coordinates the reactiveness of photosensitive functional linkages and silver cations during in situ reductions of AgNPs. Thus, visible light mediated AgNPs might be a good alternative to progress as antimicrobial agents against multiresistant pathogens.