New approach to create a counting grid by ion-mask implantation for analysis of small biological objects

Evtugin V., Rogov A., Nuzhdin V., Valeev V., Kavetskyy T., Xəlilov R., ...More

Vacuum, vol.165, pp.320-323, 2019 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 165
  • Publication Date: 2019
  • Doi Number: 10.1016/j.vacuum.2019.04.044
  • Journal Name: Vacuum
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.320-323
  • Keywords: Counting biological grid, Mask-ion implantation, Silver nanoparticles
  • Open Archive Collection: Article
  • Azerbaijan State University of Economics (UNEC) Affiliated: Yes

Abstract

A new method of forming substrates (devices) with periodic micron surface grids for statistical analysis and visual characterization of ultra-small biological objects and microorganisms is suggested. For this aim an implantation by Ar+ of silicate glasses through surface masks as wire meshes is used to form surface periodic structures in the form of grating with cell sizes of 25 μm and a depth of 100 nm. Testing of novel types of devices was carried out applying scanning electron and atomic force microscopy, as well as EDX analysis using substrates with deposited bacteria as Bacillus subtilis.